mouse polyclonal anti engrailed 1 en 1 Search Results


mouse igg1 anti engrailed 1  (Developmental Studies Hybridoma Bank)
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gene exp en1 mm00438709 m1  (Thermo Fisher)
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en 1  (Santa Cruz Biotechnology)
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mouse polyclonal anti engrailed 1 en 1  (Developmental Studies Hybridoma Bank)
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Mouse Polyclonal Anti Engrailed 1 En 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti en 1  (Developmental Studies Hybridoma Bank)
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mouse anti-engrailed-1 (en-1)  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank mouse anti-engrailed-1 (en-1)
Mouse Anti Engrailed 1 (En 1), supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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engrailed1-cre heterozygous male mice  (Jackson Laboratory)
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Jackson Laboratory engrailed1-cre heterozygous male mice
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gene exp en1 hs00154977 m1  (Thermo Fisher)
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en-1  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank en-1
Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice . Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7 -/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7 + cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and <t>Engrailed</t> <t>(En-1)</t> (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc , cerebral cortex; Ipf , interpeduncular fossa; Mes , mesencephalon; Pt , pretectum; Ri , rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.
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4d9  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank 4d9
Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice . Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7 -/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7 + cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and <t>Engrailed</t> <t>(En-1)</t> (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc , cerebral cortex; Ipf , interpeduncular fossa; Mes , mesencephalon; Pt , pretectum; Ri , rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.
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4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase  (Unigene)
90
Unigene 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice . Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7 -/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7 + cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and <t>Engrailed</t> <t>(En-1)</t> (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc , cerebral cortex; Ipf , interpeduncular fossa; Mes , mesencephalon; Pt , pretectum; Ri , rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.
4 Hydroxy 3 Methylbut 2 En 1 Yl Diphosphate Synthase, supplied by Unigene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice . Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7 -/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7 + cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and Engrailed (En-1) (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc , cerebral cortex; Ipf , interpeduncular fossa; Mes , mesencephalon; Pt , pretectum; Ri , rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.

Journal: BMC Developmental Biology

Article Title: Pax7 is requisite for maintenance of a subpopulation of superior collicular neurons and shows a diverging expression pattern to Pax3 during superior collicular development

doi: 10.1186/1471-213X-8-62

Figure Lengend Snippet: Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice . Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7 -/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7 + cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and Engrailed (En-1) (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc , cerebral cortex; Ipf , interpeduncular fossa; Mes , mesencephalon; Pt , pretectum; Ri , rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.

Article Snippet: All tissue sections were treated with 0.2% Triton-X100/PBS (10 min), 1.5% H 2 O 2 /PBS (2–3 × 10 min), blocked with 10% fetal calf serum/PBS (30 min) and incubated overnight at 4°C with primary antibodies; Pax7 (1:20 or 1:10 (E12.5), mouse, monoclonal, DSHB, Iowa City, IA, USA); Pax3 (1:100 or 1:50 (E12.5), mouse, monoclonal, DSHB, Iowa City, IA, USA); Pax6 (1:100, mouse, monoclonal, DSHB, Iowa City, IA, USA); En-1 (1:50, mouse, monoclonal, DSHB, Iowa City, IA, USA); ephrin-A2 (1:500, rabbit, polyclonal, Santa Cruz Biotechnology, Santa Cruz, CA, USA); NeuN (1:100, mouse, monoclonal, Chemicon/Millipore, Billerica, MA, USA).

Techniques: Mutagenesis, Positive Control, Expressing, Immunohistochemical staining